Elongation factor 1-gamma Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:400 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:400 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:400 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:400 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:400 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:400 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:400 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:400 for 30 minutes at room temperature, DAB staining.

$MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Elongation factor 1-gamma) monoclonal Antibody, Unconjugated (bsm-54719R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Elongation factor 1-gamma) monoclonal Antibody, Unconjugated (bsm-54719R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$PANC-1 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Elongation factor 1-gamma) monoclonal Antibody, Unconjugated (bsm-54719R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

PANC-1 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Elongation factor 1-gamma) monoclonal Antibody, Unconjugated (bsm-54719R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$SKOV-3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Elongation factor 1-gamma) monoclonal Antibody, Unconjugated (bsm-54719R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

SKOV-3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Elongation factor 1-gamma) monoclonal Antibody, Unconjugated (bsm-54719R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Daudi cells were fixed with 4% PFA for 10min at room temperature,permeabilized with for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Elongation factor 1-gamma Monoclonal Antibody(bsm-54719R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green).

Applications

WB
FCM
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54719R
Product Name	Elongation factor 1-gamma Monoclonal Antibody
Applications	WB FCM IHC-P IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within N terminal human Elongation factor 1-gamma.
Clonality	Monoclonal
Clone #	3H
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1937
Swiss Prot	P26641
Subcellular location	Cytoplasm, Nucleus, Extracellular exosome, Membrane
Synonyms	2610301D06Rik antibody, AA407312 antibody, eEF 1B gamma antibody, EEF 1G antibody, eEF-1B gamma antibody, EEF1G antibody, EF 1 gamma antibody, EF 1G antibody, EF-1-gamma antibody, EF1 gamma antibody, EF1G antibody, EF1G_HUMAN antibody, Elongation factor 1 gamma antibody, Elongation factor 1-gamma antibody, Eukaryotic translation elongation factor 1 gamma antibody, GIG 35 antibody, GIG35 antibody, MGC103354 antibody, MGC114210 antibody, MGC144723 antibody, MGC144724 antibody, MGC94929 antibody, Pancreatic tumor related protein antibody, PRO1608 antibody, Translation elongation factor eEF 1 gamma chain antibody
Background	EF-1 (elongation factor-1) is a multi-protein complex that is responsible for the delivery of aminoacyl-tRNAs to the ribosome. EF-1 gamma (elongation factor 1-gamma), also known as EEF1G or GIG35, is a 437 amino acid subunit of the EF-1 complex. Expressed in stomach, pancreas, brain, lung, kidney, intestine, liver and spleen, EF-1 gamma contains an N-terminal glutathione transferase domain which is thought to be involved in anchoring the complex to various cellular components. Additionally, EF-1 gamma may play a key role in the assembly of multiprotein complexes containing aminoacyl-tRNA synthetases. Increased expression of EF-1 gamma is associated with pancreatic cancer, suggesting a possible role for EF-1 gamma in the oncogenic transformation process.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200