phospho-Histone H3 (Ser10) monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at\r\nroom temperature\r\nSecondary Ab: Goat Anti-Mouse IgG H&L\r\n(HRP)\r\nLysate: (-) HeLa, (+) HeLa+ Nocodazole\r\n(100ng\/ml, 18hr) + Calyculin A (100nM, 1hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at\r\nroom temperature\r\nSecondary Ab: Goat Anti-Mouse IgG H&L\r\n(HRP)\r\nLysate: (-) HeLa, (+) HeLa+ Nocodazole\r\n(100ng\/ml, 18hr) + Calyculin A (100nM, 1hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa

Applications

WB
IHC-P

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-60078M
Product Name	phospho-Histone H3 (Ser10) monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser10
Clonality	Monoclonal
Clone #	A1F11
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8350
Swiss Prot	P68431
Subcellular location	Nucleus
Synonyms	Histone H3 (phospho S10); Phospho-Histone H3(Ser10); Histone H3(Phospho-Ser10); Histone H3(p-Ser10); Histone H3(Phospho-S10); H3S10ph; PHH3; H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; H31_HUMAN; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l.
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Application Dilution
WB	=1:1000-2000
IHC-P	IHC-P=1:400-800

Applications

Reactivity

Predicted Reactivity

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