ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.", "Category"=>"Primary Antibody", "Clonality"=>"Monoclonal", "Clone Number"=>"B4F3", "Concentration"=>"1mg/ml", "Conjugation"=>"Unconjugated", "Cross Reactive Species"=>"Human", "Datasheet URL"=>"https://www.biossusa.com/store/datasheets/bsm-60222R", "Gene ID"=>"4436", "Gene ID Link"=>"Entrez Gene:
4436", "Host"=>"Rabbit", "images"=>"[{\"id\":\"39242\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"5\",\"origin_notes\":\"\",\"product_id\":\"20433\",\"productconjugation_id\":null,\"application_id\":\"1\",\"application_note\":\"\",\"dilution\":\"1:1000\",\"cross_reactive_species_id\":\"1\",\"cross_reactive_species_note\":\"\",\"description\":\"Blocking buffer: 5% NFDM\\/TBST\\r\\nPrimary ab dilution: 1:1000\\r\\nPrimary ab incubation condition: 2 hours at\\r\\nroom temperature\\r\\nLysate: HeLa\\r\\nProtein loading quantity: 20 \\u03bcg\\r\\nExposure time: 60 s\\r\\nPredicted MW: 105 kDa\\r\\nObserved MW: 104 kDa\",\"sample_type_id\":\"1\",\"sample_details\":null,\"rating\":\"3\",\"disease_id\":null,\"sample_tissue_id\":null,\"cell_line_id\":\"13\",\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/39242_raw.jpg\",\"filepath_med\":\"20433\\/39242_med.jpg\",\"filepath_thumb\":\"20433\\/39242_thumb.jpg\",\"display_order\":\"1\",\"lock_version\":\"1\",\"created_at\":\"2022-02-23 03:18:39\",\"updated_at\":\"2022-02-23 03:18:39\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/39242\"},{\"id\":\"39500\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"5\",\"origin_notes\":\"\",\"product_id\":\"20433\",\"productconjugation_id\":null,\"application_id\":\"4\",\"application_note\":\"\",\"dilution\":\"1\\/50\",\"cross_reactive_species_id\":\"1\",\"cross_reactive_species_note\":\"\",\"description\":\"Tissue: Human tonsil\\r\\nSection type: Formalin fixed & Paraffin -embedded section\\r\\nRetrieval method: High temperature and high pressure\\r\\nRetrieval buffer: Tris\\/EDTA buffer, pH 9.0 Primary ab dilution: 1:50\\r\\nPrimary ab incubation condition: 1 hour at room temperature\\r\\nCounter stain: Hematoxylin\\r\\nComment: Color brown is the positive signal for bsm-60222R\",\"sample_type_id\":\"2\",\"sample_details\":null,\"rating\":\"2\",\"disease_id\":\"2\",\"sample_tissue_id\":\"22\",\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/39500_raw.jpg\",\"filepath_med\":\"20433\\/39500_med.jpg\",\"filepath_thumb\":\"20433\\/39500_thumb.jpg\",\"display_order\":\"2\",\"lock_version\":\"1\",\"created_at\":\"2022-03-07 06:56:02\",\"updated_at\":\"2022-03-07 06:56:02\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/39500\"},{\"id\":\"39501\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"5\",\"origin_notes\":\"\",\"product_id\":\"20433\",\"productconjugation_id\":null,\"application_id\":\"4\",\"application_note\":\"\",\"dilution\":\"1\\/50\",\"cross_reactive_species_id\":\"1\",\"cross_reactive_species_note\":\"\",\"description\":\"Tissue: Human esophagus\\r\\nSection type: Formalin fixed & Paraffin -embedded section\\r\\nRetrieval method: High temperature and high pressure\\r\\nRetrieval buffer: Tris\\/EDTA buffer, pH 9.0 Primary ab dilution: 1:50\\r\\nPrimary ab incubation condition: 1 hour at room temperature\\r\\nCounter stain: Hematoxylin\\r\\nComment: Color brown is the positive signal for bsm-60222R\",\"sample_type_id\":\"2\",\"sample_details\":null,\"rating\":\"3\",\"disease_id\":\"2\",\"sample_tissue_id\":\"28\",\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/39501_raw.jpg\",\"filepath_med\":\"20433\\/39501_med.jpg\",\"filepath_thumb\":\"20433\\/39501_thumb.jpg\",\"display_order\":\"3\",\"lock_version\":\"1\",\"created_at\":\"2022-03-07 06:56:28\",\"updated_at\":\"2022-03-07 06:56:29\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/39501\"},{\"id\":\"42735\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"1\",\"origin_notes\":\"\",\"product_id\":\"20433\",\"productconjugation_id\":null,\"application_id\":\"1\",\"application_note\":\"\",\"dilution\":\"1:1000\",\"cross_reactive_species_id\":\"1\",\"cross_reactive_species_note\":\"\",\"description\":\"Lane 1: Human HeLa cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human K562 cell lysates probed with MSH2 Monoclonal Antibody, Unconjugated (bsm-60222R) at 1:1000 dilution and 4\\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\",\"sample_type_id\":\"1\",\"sample_details\":null,\"rating\":\"3\",\"disease_id\":null,\"sample_tissue_id\":null,\"cell_line_id\":\"13\",\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/42735_raw.jpg\",\"filepath_med\":\"20433\\/42735_med.jpg\",\"filepath_thumb\":\"20433\\/42735_thumb.jpg\",\"display_order\":\"4\",\"lock_version\":\"1\",\"created_at\":\"2022-11-03 10:40:03\",\"updated_at\":\"2022-11-03 10:40:03\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/42735\"}]", "Isotype"=>"IgG", "Lead Time"=>"3 to 5 business days.", "Modification"=>"Unmodified", "Notes"=>"PTM-5034", "Product URL"=>"https://www.biossusa.com/products/bsm-60222R", "Purification"=>"Purified by Protein A.", "Size"=>"100ul", "Source"=>"KLH conjugated synthetic peptide derived from human MSH2", "Storage"=>"0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.", "Storage Buffer"=>"0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.", "Storage Condition"=>"Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.", "Subcellular Locations"=>"Nucleus", "Swiss Prot"=>"P43246", "Swiss Prot Link"=>"Swiss Prot:
P43246", "Synonyms"=>"MSH-2; BAT26; DNA mismatch repair protein Msh2; FCC1; COCA1; HNPCC; LCFS2; HNPCC1; BAT26; COCA 1; COCA1; DNA mismatch repair protein Msh2; FCC 1; FCC1; hMSH2; HNPCC 1; HNPCC; HNPCC1; LCFS2; MSH 2; Msh2; MSH2_HUMAN; MutS homolog 2; MutS homolog 2 colon cancer nonpolyposis type 1; MutS protein homolog 2.", "Target Protein"=>"MSH2"} -->
| Overview |
| bsm-60222R |
| MSH2 monoclonal Antibody |
| WB, IHC-P, IF, ICC |
| Human |
| Specifications |
| Unconjugated |
| Rabbit |
| KLH conjugated synthetic peptide derived from human MSH2 |
| Monoclonal |
| B4F3 |
| IgG |
| 1mg/ml |
| Purified by Protein A. |
| 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target |
| 4436 |
| P43246 |
| Nucleus |
| MSH-2; BAT26; DNA mismatch repair protein Msh2; FCC1; COCA1; HNPCC; LCFS2; HNPCC1; BAT26; COCA 1; COCA1; DNA mismatch repair protein Msh2; FCC 1; FCC1; hMSH2; HNPCC 1; HNPCC; HNPCC1; LCFS2; MSH 2; Msh2; MSH2_HUMAN; MutS homolog 2; MutS homolog 2 colon cancer nonpolyposis type 1; MutS protein homolog 2. |
| Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. |
| Application Dilution |
| WB |
=1:500-1000 |
| IHC-P |
IHC-P=1:50 |
| IF |
ICC=1:50-100 |
| ICC |
IF=1:50-100 |
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