CAPG Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:10000\r\nPrimary Ab incubation condition: room temperature 2h\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: Jurkat, 3: Raw264.7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 3 s\r\nPredicted MW: 40 kDa\r\nObserved MW: 40 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:10000\r\nPrimary Ab incubation condition: room temperature 2h\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: Jurkat, 3: Raw264.7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 3 s\r\nPredicted MW: 40 kDa\r\nObserved MW: 40 kDa

$Lane 1: Mouse Raw264.7 cell lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Jurkat cell lysates probed with CAPG Monoclonal Antibody, Unconjugated (bsm-60451M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Raw264.7 cell lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Jurkat cell lysates probed with CAPG Monoclonal Antibody, Unconjugated (bsm-60451M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Raw264.7 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates probed with CAPG Monoclonal Antibody, Unconjugated (bsm-60451M) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Raw264.7 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates probed with CAPG Monoclonal Antibody, Unconjugated (bsm-60451M) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-60451M
Product Name	CAPG Monoclonal Antibody
Applications	WB, ICC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Clonality	Monoclonal
Clone #	G11D9
Isotype	IgG1/Kappa
Concentration	1mg/ml
Purification	Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P40121
Application Dilution
WB	1:2000-10000
ICC	1:100-500

Applications

Reactivity

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