EIF3L Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:10000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate:1: 293, 2: Raw264.7, 3: 3T3, 4: HepG2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 67 kDa\r\nObserved MW: 55-70 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:10000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate:1: 293, 2: Raw264.7, 3: 3T3, 4: HepG2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 67 kDa\r\nObserved MW: 55-70 kDa

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-60479M
Product Name	EIF3L Monoclonal Antibody
Applications	WB, ICC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Clonality	Monoclonal
Clone #	B5C12
Isotype	IgG2A/Kappa
Concentration	1mg/ml
Purification	Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	Q9Y262
Application Dilution
WB	1:300-5000
ICC	1:100-500

Applications

Reactivity

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