phospho-TBK1 (Ser172) Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate:(-): HeLa, (+): HeLa+ Okadaic A+ Calyculin A (100 nM, 60min) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 84 kDa Observed MW: 84 kDa$

Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate:(-): HeLa, (+): HeLa+ Okadaic A+ Calyculin A (100 nM, 60min) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 84 kDa Observed MW: 84 kDa

$Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: (-): C6, (+): C6+Calyculin A(100nM, 30min) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 84 kDa Observed MW: 84 kDa$

Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: (-): C6, (+): C6+Calyculin A(100nM, 30min) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 84 kDa Observed MW: 84 kDa

Applications

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60659R
Product Name	phospho-TBK1 (Ser172) Monoclonal Antibody
Applications	WB
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human phospho-TBK1 (Ser172)
Modification Site	Ser172
Clonality	Monoclonal
Clone #	R7D3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	29110
Swiss Prot	Q9UHD2
Subcellular location	Cytoplasm, Nucleus
Synonyms	EC 2.7.11.1; FLJ11330; NAK; NF kappa B activating kinase; NF kB activating kinase; NF-kappa-B-activating kinase; Serine/threonine protein kinase TBK 1; Serine/threonine protein kinase TBK1; Serine/threonine-protein kinase TBK1; T2K; TANK binding kinase 1; TANK-binding kinase 1; TBK 1; TBK1_HUMAN.
Application Dilution
WB	1:300-5000

Applications

Predicted Reactivity

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