CD1c Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: THP-1 (Negative control), 2: MOLT-4, 3: Jurkat Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 38 kDa Observed MW: 43 kDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: THP-1 (Negative control), 2: MOLT-4, 3: Jurkat Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 38 kDa Observed MW: 43 kDa

$Tissue: Human tonsil Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60660R$

Tissue: Human tonsil Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60660R

$Tissue: Human spleen Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60660R$

Tissue: Human spleen Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60660R

Applications

WB
IHC-P
IHC-F

Predicted Reactivity

Human

Overview
Catalog #	bsm-60660R
Product Name	CD1c Monoclonal Antibody
Applications	WB, IHC-P, IHC-F
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD1c
Clonality	Monoclonal
Clone #	R6C8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	911
Swiss Prot	P29017
Subcellular location	Cell membrane
Synonyms	CD1c antigen; CD1C antigen c polypeptide; CD1c molecule; CD1C_HUMAN; Cortical thymocyte antigen CD1C; Differentiation antigen CD1 alpha 3 antibody R7; T cell surface glycoprotein CD1c; T-cell surface glycoprotein CD1c.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500

Applications

Predicted Reactivity

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