phospho-eIF4EBP1 (Thr37 / Thr46) Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: (-): HEK-293, (+): HEK-293 + EGF (200 \r\nng\/mL, 10 min)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 13 kDa\r\nObserved MW: 15 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: (-): HEK-293, (+): HEK-293 + EGF (200 \r\nng\/mL, 10 min)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 13 kDa\r\nObserved MW: 15 kDa

Applications

Overview
Catalog #	bsm-60678R
Product Name	phospho-eIF4EBP1 (Thr37 / Thr46) Monoclonal Antibody
Applications	WB
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human phospho-eIF4EBP1 (Thr37 / Thr46)
Modification Site	Thr37 / Thr46
Clonality	Monoclonal
Clone #	R2D8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1978
Swiss Prot	Q13541
Subcellular location	Cytoplasm
Synonyms	Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1 (PHAS-I); 4EBP1_HUMAN.
Application Dilution
WB	1:300-5000

Applications

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