EEF1A1 Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: MCF7, 2: PANC-1, 3: Neuro-2a, 4: Rat kidney Protein loading quantity: 20 \u03bcg Exposure time: 10 s Predicted MW: 50 kDa Observed MW: 55 kDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: MCF7, 2: PANC-1, 3: Neuro-2a, 4: Rat kidney Protein loading quantity: 20 \u03bcg Exposure time: 10 s Predicted MW: 50 kDa Observed MW: 55 kDa

Applications

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60686R
Product Name	EEF1A1 Monoclonal Antibody
Applications	WB, IF, ICC
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human eEF1a1
Clonality	Monoclonal
Clone #	R8F9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1915
Swiss Prot	P68104
Subcellular location	Cytoplasm
Synonyms	Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu (EF-Tu); Eukaryotic elongation factor 1 A-1 (eEF1A-1); Leukocyte receptor cluster member 7; EF1A1_HUMAN; EEF1A; EF1A; LENG7;
Application Dilution
WB	1:300-5000
IF
ICC	1:100-500

Applications

Predicted Reactivity

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