PRAME Recombinant Antibody

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4\u2103\r\novernight Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: Ramos, 2: K562, 3: HeLa, 4: A375 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 58 kDa Observed MW: 58 kDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4\u2103\r\novernight Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: Ramos, 2: K562, 3: HeLa, 4: A375 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 58 kDa Observed MW: 58 kDa

Applications

Reactivity

Human

Overview
Catalog #	bsm-60694r
Product Name	PRAME Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PRAME
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23532
Swiss Prot	P78395
Subcellular location	Nucleus, Cell membrane
Synonyms	MAPE; Melanoma antigen preferentially expressed in tumors; OIP 4; OIP-4; OIP4; OPA interacting protein 4; Opa interacting protein OIP4; OPA-interacting protein 4; PRAME_HUMAN; Preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma; RP23-250F8.3.
Application Dilution
WB	1:300-5000