CHD3 Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: C6, 2: HeLa Protein loading quantity: 20 \u03bcg Exposure time: 1: 60 s, 2: 30 s Predicted MW: 227 kDa Observed MW: 260 kDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: C6, 2: HeLa Protein loading quantity: 20 \u03bcg Exposure time: 1: 60 s, 2: 30 s Predicted MW: 227 kDa Observed MW: 260 kDa

$Tissue: Human breast Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60708R\r\n$

Tissue: Human breast Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60708R\r\n

Applications

WB
IHC-P
IHC-F
IF
ICC

Predicted Reactivity

Human
Rat

Overview
Catalog #	bsm-60708R
Product Name	CHD3 Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF, ICC
Predicted Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CHD3
Clonality	Monoclonal
Clone #	R3C6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1107
Swiss Prot	Q12873
Subcellular location	Cytoplasm, Nucleus
Synonyms	CHD3_HUMAN; Chromodomain-helicase-DNA-binding protein 3; EC:3.6.4.12; CHD-3; ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase (hZFH);
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF
ICC	1:100-500

Applications

Predicted Reactivity

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