PDCD6IP Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:10000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: HeLa, 2: C6, 3: Mouse brain, 4: Rat \r\nbrain\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 96 kDa\r\nObserved MW: 90\/100 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:10000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: HeLa, 2: C6, 3: Mouse brain, 4: Rat \r\nbrain\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 96 kDa\r\nObserved MW: 90\/100 kDa

$Tissue: Human breast cancer\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60716R$

Tissue: Human breast cancer\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60716R

Applications

WB
IHC-P
IHC-F

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60716R
Product Name	PDCD6IP Monoclonal Antibody
Applications	WB, IHC-P, IHC-F
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PDCD6IP
Clonality	Monoclonal
Clone #	R4B8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10015
Swiss Prot	Q8WUM4
Subcellular location	Cytoplasm
Synonyms	PDC6I_HUMAN; Programmed cell death 6-interacting protein; AIP1; ALIX; KIAA1375; PDCD6-interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95;
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500

Applications

Predicted Reactivity

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