Free shipping on your order over $1,000! Offer valid on U.S. domestic orders only.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

FTO Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (bsm-60733R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (bsm-60733R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (bsm-60733R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (bsm-60733R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (bsm-60733R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (bsm-60733R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Human SW480 cell lysates probed with FTO Monoclonal Antibody, Unconjugated (bsm-60733R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Human SW480 cell lysates probed with FTO Monoclonal Antibody, Unconjugated (bsm-60733R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • IHC-P

Reactivity

  • Human
Overview
Catalog # bsm-60733R
Product Name FTO Monoclonal Antibody
Applications WB, IHC-P
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human FTO
Clonality Monoclonal
Clone # -
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 79068
Swiss Prot Q9C0B1
Synonyms Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; FTO_HUMAN; Protein fatso.
Application Dilution
WB =1:1000
IHC-P IHC-P=1:100-500

Search our catalog of hundreds of thousands of products