TLR4 Monoclonal Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with TLR4 monoclonal antibody, unconjugated (bsm-60911M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with TLR4 monoclonal antibody, unconjugated (bsm-60911M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Mouse

Overview
Catalog #	bsm-60911m
Product Name	TLR4 Monoclonal Antibody
Applications	WB
Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human TLR4
Clonality	Monoclonal
Clone #	1G12
Isotype	IgG1
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7099
Swiss Prot	O00206
Subcellular location	Cytoplasm, Cell membrane
Synonyms	TLR-4; TLR 4; ARMD10; CD 284; CD284; CD284 antigen; Homolog of Drosophila toll; hTol; Toll (Drosophila) homolog; TOLL; Toll Endotoxin Hyporesponsiveness; Toll like receptor 4; Toll like receptor 4 precursor; TLR4_HUMAN.
Background	Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
Application Dilution
WB	1:500-2000