RALBP1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with RALBP1 monoclonal antibody, unconjugated (bsm-61265R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with RALBP1 monoclonal antibody, unconjugated (bsm-61265R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Stomach;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Stomach;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Stomach;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Stomach;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Stomach;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Stomach;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Liver;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RALBP1 Monoclonal Antibody, Unconjugated (bsm-61265R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
FCM
IHC-P
IHC-F
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61265r
Product Name	RALBP1 Recombinant Antibody
Applications	WB FCM IHC-P IHC-F IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RalBP1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10928
Swiss Prot	Q15311
Subcellular location	Cytoplasm
Synonyms	RALBP1; RIP1; RLIP1; RLIP76.
Background	Multifunctional protein that functions as a downstream effector of RALA and RALB.As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity.As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100
IHC-P	IHC-P1:100-500
IHC-F	IHC-F1:100-500
IF(IHC-P)	IFIHC-P1:100-500