HSPA4 Recombinant Antibody

$Western blot analysis of HepG2 cell lysate. Using HSPA4 (bsm-61343R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of HepG2 cell lysate. Using HSPA4 (bsm-61343R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61343r
Product Name	HSPA4 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Hsp70 4
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3308
Swiss Prot	P34932
Subcellular location	Cytoplasm
Synonyms	HSPA4; APG-2; APG2; Heat shock 70kD protein 4; Hsp70RY; HSPH2; Hsp70; HS24/P52; Heat shock 70kDa protein 4; Heat shock protein 110 kDa.
Background	The heat shock protein HSPA4 (Apg-2, HSP70RY) is a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 heat-shock proteins. Apg-2 has chaperone ability similiar to Hsp110, and it plays a role under non-stress conditions. Apg-2 interacts with TJP1/ZO-1 and functions as a regulator of ZO-1-ZONAB signaling in epithelial cells in response to cellular stress.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF
Flow-Cyt	1ug/Test
ICC/IF	1:50-100