Rhodopsin Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat Eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Rhodopsin Monoclonal Antibody, Unconjugated (bsm-61439R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Rhodopsin Monoclonal Antibody, Unconjugated (bsm-61439R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Rat

Overview
Catalog #	bsm-61439r
Product Name	Rhodopsin Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Rhodopsin
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6010
Swiss Prot	P08100
Subcellular location	Cell membrane
Synonyms	CSNBAD1; OPN2; opsd; Opsin 2 rod pigment; Opsin2; Retinitis pigmentosa 4 autosomal dominant; RHO; Rhodopsin; RP4.
Background	Photoreceptor required for image-forming vision at low light intensity.Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF