GCLC Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with GCLC monoclonal antibody, unconjugated (bsm-61478R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with GCLC monoclonal antibody, unconjugated (bsm-61478R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Predicted Reactivity

Overview
Catalog #	bsm-61478r
Product Name	GCLC Recombinant Antibody
Applications	WB
Reactivity	Human
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GCLC
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2729
Swiss Prot	P48506
Subcellular location	Cytoplasm
Synonyms	GCS heavy chain; Gamma-ECS Gamma-glutamylcysteine synthetase; GLCL; GLCLC; GCL.
Background	Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis.
Application Dilution
WB	1:300-5000