BubR1 Recombinant Antibody

$Western blot analysis of HepG2 cell lysate. Using BubR1 (bsm-61498R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of HepG2 cell lysate. Using BubR1 (bsm-61498R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IP
IF
Flow-Cyt

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61498r
Product Name	BubR1 Recombinant Antibody
Applications	WB IHC-P IHC-F IP IF FLOW-CYT
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human BUB1B
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	701
Swiss Prot	O60566
Subcellular location	Cytoplasm, Nucleus
Synonyms	hBUBR1; Mitotic checkpoint kinase MAD3L Protein SSK1; BUB1B; BUBR1; MAD3L; SSK1.
Background	Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IP	1-2ug
IF
Flow-Cyt	1ug/Test