Phospho-Crkl (Tyr207) Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Phospho-Crkl (Tyr207) monoclonal antibody, unconjugated (bsm-61642R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Phospho-Crkl (Tyr207) monoclonal antibody, unconjugated (bsm-61642R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$The K562 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Phospho-Crkl (Tyr207) antibody (bsm-61642R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The K562 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Phospho-Crkl (Tyr207) antibody (bsm-61642R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

Reactivity

Human

Overview
Catalog #	bsm-61642r-TR
Product Name	Phospho-Crkl (Tyr207) Recombinant Antibody
Applications	WB, FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CRKL around the phosphorylation site of Y207
Modification Site	Tyr207
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1399
Swiss Prot	P46109
Subcellular location	Cytoplasm, Nucleus
Synonyms	Crk L; Crk like protein; Crkl; Crkol; Oncogene CrkL.
Background	May mediate the transduction of intracellular signals.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100