KMT5A Recombinant Antibody

$The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-KMT5A antibody (bsm-61681R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-KMT5A antibody (bsm-61681R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61681r-TR
Product Name	KMT5A Recombinant Antibody
Applications	WB FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human KMT5A
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	387893
Swiss Prot	Q9NQR1
Subcellular location	Nucleus
Synonyms	KMT5A;PR-Set7;SET07;SET8.
Background	Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins.Specifically monomethylates 'Lys-20' of histone H4.H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression.
Application Dilution
WB	1:1000-2000
FCM	FCM1:50-100