PU.1/Spi1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with PU.1\/Spi1 monoclonal antibody, unconjugated (bsm-61756R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with PU.1\/Spi1 monoclonal antibody, unconjugated (bsm-61756R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-61756r
Product Name	PU.1/Spi1 Recombinant Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SPI1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6688
Swiss Prot	P17947
Subcellular location	Nucleus
Synonyms	oncogene spi1; PU.1; SFPI1; SPI 1; SPI A; Spi1.
Background	Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions.
Application Dilution
WB	1:500-2000