Phospho-GCN2 (Thr899) Recombinant Antibody

$Western blot analysis of (1) HeLa cell lysate; (2) HeLa cell lysate treated with Calyculin. Using Phospho-GCN2 (Thr899) (bsm-61819R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) HeLa cell lysate; (2) HeLa cell lysate treated with Calyculin. Using Phospho-GCN2 (Thr899) (bsm-61819R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

Reactivity

Human

Overview
Catalog #	bsm-61819r
Product Name	Phospho-GCN2 (Thr899) Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GCN2 around the phosphorylation site of T899
Modification Site	Thr899
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	440275
Swiss Prot	Q9P2K8
Synonyms	GCN2-like protein; GCN2; KIAA1338; EIF2AK4.
Background	Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability.Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation.
Application Dilution
WB	1:300-5000