PKC theta Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with PKC theta monoclonal antibody, unconjugated (bsm-61824R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with PKC theta monoclonal antibody, unconjugated (bsm-61824R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bsm-61824r
Product Name	PKC theta Recombinant Antibody
Applications	WB
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PKC theta
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5588
Swiss Prot	Q04759
Synonyms	nPKC theta; nPKCtheta; PKC0; Prkcq; PRKCT.
Background	Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters.
Application Dilution
WB	1:500-2000