Legumain Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Legumain monoclonal antibody, unconjugated (bsm-62150R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Legumain monoclonal antibody, unconjugated (bsm-62150R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62150r
Product Name	Legumain Recombinant Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Legumain
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5641
Swiss Prot	Q99538
Subcellular location	Cytoplasm
Synonyms	AEP; cysteine 1; Legumain; LGMN; LGMN1; PRSC1.
Background	Has a strict specificity for hydrolysis of asparaginyl bonds.Can also cleave aspartyl bonds slowly, especially under acidic conditions.Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
Application Dilution
WB	1:500-2000