NCF1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with NCF1 monoclonal antibody, unconjugated (bsm-62199R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with NCF1 monoclonal antibody, unconjugated (bsm-62199R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-62199r
Product Name	NCF1 Recombinant Antibody
Applications	WB IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human p47 phox
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	653361
Swiss Prot	P14598
Subcellular location	Cytoplasm
Synonyms	NCF1; NCF1A; NOXO2; SH3PXD1A; p47phox.
Background	NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Application Dilution
WB	1:1000-2000
IP	IP1:20-50