Engrailed 1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Engrailed 1 monoclonal antibody, unconjugated (bsm-62208R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Engrailed 1 monoclonal antibody, unconjugated (bsm-62208R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bsm-62208r
Product Name	Engrailed 1 Recombinant Antibody
Applications	WB FCM IP
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human EN1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2019
Swiss Prot	Q05925
Subcellular location	Nucleus
Synonyms	EN1; Engrailed 1; Engrailed; Hu-En-1; Mo-en.1.
Background	Belongs to the engrailed homeobox family.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100
IP	IP1:20-50