LHX3 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with LHX3 monoclonal antibody, unconjugated (bsm-62259R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with LHX3 monoclonal antibody, unconjugated (bsm-62259R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62259r
Product Name	LHX3 Recombinant Antibody
Applications	WB FCM IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human LHX3
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8022
Swiss Prot	Q9UBR4
Subcellular location	Nucleus
Synonyms	CPHD3; LHX3; Lim3; M2 LHX3; mLim-3; mLIM3; P LIM.
Background	Transcription factor. Recognizes and binds to the consensus sequence motif 5'-AATTAATTA-3' in the regulatory elements of target genes, such as glycoprotein hormones alpha chain CGA and visual system homeobox CHX10, positively modulating transcription; transcription can be co-activated by LDB2. Synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100
IP	IP1:20-50