SCIN Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with SCIN monoclonal antibody, unconjugated (bsm-62533R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with SCIN monoclonal antibody, unconjugated (bsm-62533R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bsm-62533r
Product Name	SCIN Recombinant Antibody
Applications	WB
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Scinderin
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	85477
Swiss Prot	Q9Y6U3
Subcellular location	Cytoplasm
Synonyms	Adseverin; Scin; Scinderin.
Background	Ca2+-dependent actin filament-severing protein that has a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane.Severing activity is inhibited by phosphatidylinositol 4,5-bis-phosphate.In vitro, also has barbed end capping and nucleating activities in the presence of Ca2+. Required for megakaryocyte differentiation, maturation, polyploidization and apoptosis with the release of platelet-like particles.
Application Dilution
WB	1:500-2000