Phospho-Rb2 p130 (T986) Recombinant Antibody

$Western blot analysis of (1) Jurkat cell lysate; (2) Jurkat cell treated with phosphatase lysate. Using Phospho-Rb2 p130 (T986) (bsm-62720R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) Jurkat cell lysate; (2) Jurkat cell treated with phosphatase lysate. Using Phospho-Rb2 p130 (T986) (bsm-62720R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-62720r
Product Name	Phospho-Rb2 p130 (T986) Recombinant Antibody
Applications	WB IP
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RBL2 around the phosphorylation site of T986
Modification Site	Thr986
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5934
Swiss Prot	Q08999
Synonyms	130 kDa retinoblastoma associated protein; IB4; p130; PRB2; Rb2; Rbl2; RBR2; Retinoblastoma like 2; Retinoblastoma-related protein 2; RIC128.
Background	Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation.
Application Dilution
WB	1:300-5000
IP	1-2ug