PMF1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with PMF1 monoclonal antibody, unconjugated (bsm-62760R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with PMF1 monoclonal antibody, unconjugated (bsm-62760R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62760r
Product Name	PMF1 Recombinant Antibody
Applications	WB IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Polyamine modulated factor 1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	100527963
Swiss Prot	Q6P1K2
Subcellular location	Nucleus
Synonyms	Pmf1; Polyamine modulated factor 1.
Background	Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
Application Dilution
WB	1:500-2000
IP	IP1:20-50