phospho-mTOR(S2448) Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat\r\n Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat\r\n Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human\r\n Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human\r\n Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat\r\n Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat\r\n Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast \r\n Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast \r\n Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-mTOR(S2448) Monoclonal Antibody, Unconjugated (bsm-63190R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$MCF-7 (H) cells were treated with or without Camptothecin (1 \u00b5M) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-mTOR(S2448) monoclonal antibody, unconjugated (bsm-63190R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

MCF-7 (H) cells were treated with or without Camptothecin (1 \u00b5M) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-mTOR(S2448) monoclonal antibody, unconjugated (bsm-63190R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$NIH\/3T3 (M) serum-starved insulin-treated (150 nM) for 5 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-mTOR(S2448) monoclonal antibody, unconjugated (bsm-63190R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

NIH\/3T3 (M) serum-starved insulin-treated (150 nM) for 5 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-mTOR(S2448) monoclonal antibody, unconjugated (bsm-63190R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Overview
Catalog #	bsm-63190r-100l
Product Name	phospho-mTOR(S2448) Recombinant Antibody
Applications	WB IHC-P IHC-F IF(IHC-P)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human mTOR around the phosphorylation site of S2448
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2475
Swiss Prot	P42345
Subcellular location	Cytoplasm, Cell membrane
Synonyms	FRAP; FRAP1; FRAP2; RAFT1; Rapamycin target protein; kinase mTOR.
Application Dilution
WB	IFIHC-P1:50-200
IHC-P	IHC-F1:50-200
IHC-F	IHC-P1:50-200
IF(IHC-P)	WB1:500-2000