| Overview |
| bsm-70089M |
| Fibrillarin Antibody |
| WB, IHC, ICC |
| Specific for endogenous levels of the ~34 kDa Fibrillarin /Nop1p protein. |
| Human, Mouse, Rat, Bovine, Pig, Chicken, Drosophila |
| Dog, Sheep, Horse, Cat, Rabbit, Guinea Pig, Goat, Hamster, Non-Human Primate, Vole |
| Specifications |
| Unconjugated |
| Mouse |
| Yeast nuclear preparation |
| Monoclonal |
| 38F3 |
| IgG1 |
| Lot Dependent |
| Purified by Protein G. |
| PBS + 50% glycerol and 5 mm NaN3 |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| 851548 |
| P15646 |
| 34 kD nucleolar scleroderma antigen antibody, 34 kDa nucleolar scleroderma antigen antibody, Fbl antibody, FBRL_HUMAN antibody, FIB antibody, FIB1 antibody, FLRN antibody, Histone-glutamine methyltransferase antibody, Nop1p antibody, RNA U3 small nucleolar interacting protein 1 antibody, RNU3IP1 antibody, rRNA 2' O methyltransferase fibrillarin antibody, rRNA 2'-O-methyltransferase fibrillarin antibody |
| Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archaea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6). |
| Application Dilution |
| WB |
1:300-5000 |
| IHC |
|
| ICC |
1:100-500 |
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