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Mitochondria Antibody

Applications

  • WB
  • IHC

Reactivity

  • Human
Overview
Catalog # bsm-70160M
Product Name Mitochondria Antibody
Applications WB, IHC
Specificity Specific for endogenous levels of the ~60 kDa human mitochondria. The antibody works on acetone fixed and 2% paraformaldehyde fixed cells. The antibody also works for immunohistochemistry on 2% paraformaldehyde fixed (5-10 minutes) cryostat sections.
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Human cell homogenate
Clonality Monoclonal
Clone # #REF!
Isotype IgG1
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer PBS
Storage Condition Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20 C or -80 C, while keeping a working aliquot stored at 4 C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Target
Synonyms mitochondrial marker
Background Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006). In addition, mitochondria have been shown to play a role in apoptosis (Green 1998). This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Application Dilution
WB 1:300-5000
IHC