c-Src (N-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70681M |
| Product Name | c-Src (N-terminal region) Antibody |
| Applications | WB, IP |
| Specificity | This antibody detects a 60 kDa* protein corresponding to c-Src on SDS-PAGE immunoblots of mouse SYF cells transformed with c-Src and human A431 cells. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M259) was generated from a recombinant protein containing amino acids in the N-terminal region of human c-Src. This sequence has high homology to similar regions in rat and mouse c-Src. |
| Clonality | Monoclonal |
| Clone # | M259 |
| Isotype | IgG1 |
| Purification | Purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P12931 |
| Synonyms | Src |
| Background | c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. The activity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation. Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 downregulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
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