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Published Antibodies


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A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PDEF\/SPDEF Polyclonal Antibody(bs-1866R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SPDEF) Polyclonal Antibody, Unconjugated (bs-1866R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SPDEF) Polyclonal Antibody, Unconjugated (bs-1866R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SPDEF) polyclonal Antibody, Unconjugated (bs-1866R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-1866R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1375R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-0522R-BF555

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bs-0295p-bf488-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-488

Catalog #1: bs-8605R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti-FCGBP Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FCGBP Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FCGBP Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4\u00b0C, DAB staining.
Positive sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat intestine, left); Negative sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat lung, right);\r\nAntigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.\r\n

Catalog #1: bs-13168R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-LPA2 Polyclonal Antibody, Unconjugated (bs-10368R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Mouse splenocytes probed with \\tRabbit Anti-LPA2 Polyclonal Antibody, Unconjugated (bs-10368R) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary \\n(green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control orange).
Rat testis lysates probed with LPA2 Polyclonal Antibody, Unconjugated (bs-10368R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (LPA2) polyclonal Antibody, Unconjugated (bs-10368R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-10368R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7053R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-beta Defensin 1 Polyclonal Antibody, Unconjugated (bs-1163R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat kidney labeled with Anti-beta Defensin 1 Polyclonal Antibody, Unconjugated (bs-1163R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-beta defensin 1 Polyclonal Antibody, Unconjugated (bs-1163R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse trachea tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Rat trachea tissue lysates; Lane 4: Rat Kidney tissue lysates probed with Defensin beta 1 Polyclonal Antibody, Unconjugated (bs-1163R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1163R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11462R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart labeled with Anti-HCN4 Polyclonal Antibody, Unconjugated (bs-1691R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse heart lysates probed with HCN4 Polyclonal Antibody, Unconjugated (bs-1691R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Lane 1: Mouse Testis Lysates; Lane 2: Rat Testis Lysates; Lane 3: Human HL-60 cell Lysates; Lane 4: Human MCF-7 cell Lysates; Lane 5: Human A549 cell Lysates. Probed with HCN4 polyclonal Antibody, unconjugated (bs-1691R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF-7 cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with HCN4 Polyclonal Antibody(bs-1691R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-1691R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0819R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0357R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti GAD65 Polyclonal Antibody, Unconjugated (bs-0400R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti GAD65 Polyclonal Antibody, Unconjugated (bs-0400R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GAD65) polyclonal Antibody, Unconjugated (bs-0400R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Hippocampus tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates; Lane 6: Rat Hippocampus tissue lysates; Lane 7: Human SH-SY5Y cell lysates; Lane 8: Human U87MG cell lysates; Lane 9: Human HeLa cell lysates; Lane 10: Human Jurkat cell lysates probed with GAD65 Polyclonal Antibody, Unconjugated (bs-0400R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0400R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-VEGFR3 Polyclonal Antibody, Unconjugated (bs-2202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded Human Gastric Cancer labeled with VEGFR3 Polyclonal Antibody, Unconjugated (bs-2202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
A549 cells probed with \\tVEGFR3 Polyclonal Antibody, Unconjugated (bs-2202R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Formalin-fixed and paraffin-embedded Human Gastric Cancer labeled with VEGFR3 Polyclonal Antibody, Unconjugated (bs-2202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-2202R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat spleen tissue labeled with Rabbit Anti-MPL Polyclonal Antibody, Unconjugated (bs-10362R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
U2-OS cells(black) were fixed with 4% PFA for 10min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MPL Polyclonal Antibody(bs-10362R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-10362R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse spleen lysates probed with Rabbit Anti-IL-12 beta Polyclonal Antibody, Unconjugated (bs-10641R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-IL-12 beta Polyclonal Antibody, Unconjugated (bs-10641R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-10641R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-4842R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Anti-HNF4\/TCF4\/HNF4A Polyclonal Antibody, Unconjugated (bs-3828R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
BRL-3A cell lysates probed with HNF4A Polyclonal Antibody, Unconjugated (bs-3828R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hcclm3 cell lysates probed with HNF4A Polyclonal Antibody, Unconjugated (bs-3828R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3828R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-SCD1 Polyclonal Antibody, Unconjugated (bs-3787R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with SCD1 Polyclonal Antibody (bs-3787R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SCD1 Polyclonal Antibody, Unconjugated (bs-3787R) at 1:400 overnight at 4\u00b0C, DAB staining.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SCD1) polyclonal Antibody, Unconjugated (bs-3787R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-3787R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse tooth germ labeled with Anti-Oct-3\/Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
L1 rat brain lysates L2 rat heart lysates probed with Anti Oct-3\/Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 42kD. Observed band size:42kD.\\n
Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1111R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0590R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-SPRR3\/Cornifin B Polyclonal Antibody, Unconjugated (bs-11163R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
U937 cell lysates probed with SPRR3 Polyclonal Antibody, Unconjugated (bs-11163R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11163R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12313R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

F9 cells probed with Rabbit Anti-SP-C Polyclonal Antibody (bs-10067R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Formalin-fixed and paraffin embedded human prostate tissue labeled with Anti-FOXA2\/HNF 3beta Polyclonal Antibody, Unconjugated (bs-2358R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Mouse Placenta lysates probed with FOXA2 Polyclonal Antibody, Unconjugated (bs-2358R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FOXA2 Polyclonal Antibody(bs-2358R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2358R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse lymphoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tCD272 Polyclonal Antibody, Unconjugated (bs-0624R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Mouse Lymph node lysates probed with CD272 Polyclonal Antibody, Unconjugated (bs-0624R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Rat Thymus tissue lysates; Lane 2: Rat Lung tissue lysates probed with CD272 Polyclonal Antibody, Unconjugated (bs-0624R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0624R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-NSD1 Polyclonal Antibody, Unconjugated (bs-8170R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-NSD1 Polyclonal Antibody, Unconjugated (bs-8170R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NSD1 Polyclonal Antibody, Unconjugated (bs-8170R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-8170R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-5218P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450\\n
Lane 1: mouse embryo lysates Lane 2: mouse pancreas lysates probed with Anti Phospho-Wee1(Ser123)Polyclonal Antibody, Unconjugated (bs-5218R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 71kD. Observed band size: 71kD.

Catalog #1: bs-5218R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Alpha-Synuclein Polyclonal Antibody, Unconjugated (bs-0968R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Alpha-Synuclein Polyclonal Antibody, Unconjugated (bs-0968R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Alpha-Synuclein Polyclonal Antibody, Unconjugated (bs-0968R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Alpha-Synuclein Polyclonal Antibody, Unconjugated (bs-0968R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0968r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3331R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-LRIG1 Polyclonal Antibody, Unconjugated (bs-1844R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: mouse brain lysates Lane 2: mouse embryo lysates probed with Anti LRIG1 Polyclonal Antibody, Unconjugated (bs-1844R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 120kD. Observed band size: 120kD.

Catalog #1: bs-1844R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti CARD4 Polyclonal Antibody, Unconjugated (bs-7085R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti CARD4 Polyclonal Antibody, Unconjugated (bs-7085R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Raw264.7 cell lysates; Lane 2: Mouse thymus lysates probed with CARD4 Polyclonal Antibody, Unconjugated (bs-7085R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7085R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human pancreatic cancer labeled with Anti-EDG7\/LPA3 Polyclonal Antibody, Unconjugated (bs-2882R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (LPA3) Polyclonal Antibody, Unconjugated (bs-2882R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-2882R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human A549 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Hela cell lysates probed with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Antibody(bs-10011R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Polyclonal Antibody(bs-10011R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-10011R

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Pan cytokeratin Polyclonal Antibody, Unconjugated (bs-10403R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Pan cytokeratin Polyclonal Antibody, Unconjugated (bs-10403R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAPI staining
Paraformaldehyde-fixed, paraffin embedded mouse lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Pan cytokeratin Polyclonal Antibody, Unconjugated (bs-10403R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Pan cytokeratin Polyclonal Antibody, Unconjugated (bs-10403R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAPI staining

Catalog #1: bs-10403R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: human colon carcinoma lysates Lane 2: mouse brain lysates probed with Anti phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 110-123kD. Observed band size: 115,120kD.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human MCF-7 cell lysates; Lane 2: Human K562 cell lysates probed with phospho-PI3 Kinase p110 beta (Ser1070) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6417R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti SIRT3 Polyclonal Antibody, Unconjugated (bs-6105R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Liver lysates; Lane 2: Mouse Kindey lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: MouseHeart lysates probed with SIRT3 Polyclonal Antibody, Unconjugated (bs-6105R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (bs-6105R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (bs-6105R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-6105R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4801R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-4100R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart labeled with Anti LATS2 Polyclonal Antibody, Unconjugated (bs-4081R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LATS1\/2 Polyclonal Antibody, Unconjugated (bs-4081R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LATS1\/2 Polyclonal Antibody, Unconjugated (bs-4081R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LATS1\/2 Polyclonal Antibody, Unconjugated (bs-4081R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-4081R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse embryo lysates probed with Phospho-eNOS (Ser1177) Polyclonal Antibody, Unconjugated (bs-3447R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with eNOS (Ser1177) Polyclonal Antibody(bs-3447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human HUVEC cell lysates; Lane 2: Human HUVEC treated with 100 \u00b5M Pervanadate (PV) for 10 minutes lysates probed with Phospho-eNOS (Ser1177) Polyclonal Antibody, Unconjugated (bs-3447R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-3447r-TR

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse colon carcinoma labeled with Anti-NQO1 Polyclonal Antibody, Unconjugated (bs-2184R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat liver lysates L2 rat brain lysates probed with Anti NQO1 Polyclonal Antibody, Unconjugated (bs-2184R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 30kD. Observed band size:30kD.\\n
Lane 1: A549 cell lysates probed with NQO1 Polyclonal Antibody, Unconjugated (bs-2184R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human Du145 cell lysates probed with NQO1 Polyclonal Antibody, Unconjugated (bs-2184R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2184R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1761R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-STAT3 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody (bs-1141R), (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded human cervical tissue labeled with Anti-STAT3 Polyclonal Antibody (bs-1141R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
RSC96 cells probed with Rabbit Anti-STAT3 Polyclonal Antibody (bs-1141R),6ug, for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC)at 0.5ug(green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 Polyclonal Antibody, Unconjugated (bs-1141R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1141R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12359R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11514R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

L1 rat heart lysates L2 rat brain lysates probed with Anti CX3CL1 Polyclonal Antibody, Unconjugated (bs-0811R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 41kD. Observed band size:41\/70kD.
Human A549 cell lysates probed with Rabbit Anti-CX3CL1 Polyclonal Antibody, Unconjugated (bs-0811R) (green) at 1:10 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-CX3CL1 Polyclonal Antibody, Unconjugated (bs-0811R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-0811R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver tissue with Anti-Collagen IV Polyclonal Antibody, Unconjugated (bs-0806R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded rabbit cartilage tissue with Anti-Collagen IV Polyclonal Antibody, Unconjugated (bs-0806R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Recombinant human Collagen IV protein, N-His (bs-42306P) probed with Collagen IV Polyclonal Antibody, Unconjugated (bs-0806R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-0806R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated