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Published Antibodies


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Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells; Antibody incubation with S100A2 (2C1) Monoclonal Antibody, Unconjugated (bsm-51330M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A2 Monoclonal Antibody, Unconjugated (bsm-51330M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A2 Monoclonal Antibody, Unconjugated (bsm-51330M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A2 Monoclonal Antibody, Unconjugated (bsm-51330M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51330m

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: SW480 Cell lysates; probed with TIMP2 (4C7) Monoclonal Antibody, unconjugated (bsm-51320M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 cells labeled with TIMP2 (4C7) Monoclonal Antibody (bsm-51320M) at 1\/25 dilution followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
K562 cells probed with using TIMP2 (4C7) Monoclonal Antibody bsm-51320M at 1:25 for 30 minutes followed by A488 secondary antibody (green) at 1:200, compared to an isotype control of mouse IgG1 (blue).
Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Blocked with 3% BSA for 30min at RT; Antibody incubation with TIMP2 (4C7) Monoclonal Antibody, Unconjugated (bsm-51320M ) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51320M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30092M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33008m

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NCAM) polyclonal Antibody, Unconjugated (bsm-52824R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NCAM Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NCAM Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NCAM Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52824r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with TCF7L2 Monoclonal Antibody(bsm-52543R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TCF7L2 Monoclonal Antibody, Unconjugated (bsm-52543R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TCF7L2 Monoclonal Antibody, Unconjugated (bsm-52543R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TCF7L2 Monoclonal Antibody, Unconjugated (bsm-52543R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52543r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

4% Paraformaldehyde-fixed Raji (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HDAC9) monoclonal Antibody, unconjugated (bsm-54186R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with HDAC9 monoclonal antibody, unconjugated (bsm-54186R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HDAC9 Monoclonal Antibody, Unconjugated (bsm-54186R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HDAC9 Monoclonal Antibody, Unconjugated (bsm-54186R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54186r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Tyrosine Hydroxylase (8B6)) Monoclonal Antibody, Unconjugated (bsm-52574R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Tyrosine Hydroxylase (8B6)) Monoclonal Antibody, Unconjugated (bsm-52574R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tyrosine Hydroxylase Monoclonal Antibody, Unconjugated(bsm-52574R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tyrosine Hydroxylase Monoclonal Antibody, Unconjugated(bsm-52574R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52574r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with VCAM1 Monoclonal Antibody, Unconjugated (bsm-52248R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VCAM1 Monoclonal Antibody, Unconjugated(bsm-52248R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VCAM1 Monoclonal Antibody, Unconjugated(bsm-52248R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
\tParaformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VCAM1 Monoclonal Antibody, Unconjugated(bsm-52248R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52248r-TR

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Cyclin B1) monoclonal Antibody, unconjugated (bsm-52044R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with Cyclin B1 monoclonal antibody, unconjugated (bsm-52044R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-52044r-TR

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 14 (2F11) Monoclonal Antibody, Unconjugated (bsm-52054R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 14 Monoclonal Antibody, Unconjugated(bsm-52054R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 14 Monoclonal Antibody, Unconjugated(bsm-52054R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 14 Monoclonal Antibody, Unconjugated(bsm-52054R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52054r-TR

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (LC3A ) Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (LC3A ) Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33309m

Applications: WB, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-54066r-TR

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane1: HeLa lysates; Lane 2: HepG2 lysates; Lane 3: mouse placenta lysates probed with AMPK alpha-1 Polyclonal Antibody, Unconjugated (bs-33338M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1 Monoclonal Antibody, Unconjugated (bsm-33338M) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Rat Liver lysates; Lane 2: Human Hela cell lysates; Lane 3: Human HepG2 cell lysates probed with AMPK alpha-1 Monoclonal Antibody, Unconjugated (bsm-33338M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cell; 4% Paraformaldehyde-fixed; Ice-cold methanol at -20\u2103 for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (AMPK alpha-1) monoclonal Antibody, Unconjugated (bsm-33338M) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33338M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52136r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

SH-SY-5Y cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with eIF4E(S209) (2B1 ) Monoclonal Antibody (bsm-52152R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Lane 1: Mouse Pancreas tissue lysates; Lane 2:Rat Lymph node tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Pancreas tissue lysates ; Lane 5: Human Hela cell lysates;Lane 6: Human 293T cell lysates ;Lane 7: Human MCF-7 cell lysates; Lane 8: Human SH-SY5Y cell lysates probed with eIF4E(S209) (2B1) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with eIF4E(S209) (2B1) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with eIF4E(S209) (2B1) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52152r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: MCF-7 Cell lysates; Lane 2: U266 Cell lysates; probed with CDKN1B (10B7) Monoclonal Antibody, unconjugated (bsm-51281M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDKN1B (10B7) Monoclonal Antibody (bsm-51281M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51281M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: PC-12 probed with TOP1 (1C6) Monoclonal Antibody, unconjugated (bsm-51396M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells stained with TOP1 (1C6) Monoclonal Antibody, bsm-51396M at 1:25 dilution, followed by Alexa Fluor\u00ae 488 goat anti-mouse lgG (green), compared to mouse IgG1 isotype control (blue).
Lane 1: Molt-4 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Siha cell lysates; Lane 4: K562 cell lysates probed with TOP1 (1C6) Monoclonal Antibody, Unconjugated (bsm-51396M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51396M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : MCF7 Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : C6 Cell Lysate; Lane 4 : L929 Cell Lysate; probed with using bsm-50375M at Cyclin D1 (2G5) Monoclonal Antibody 4.0ug\/mL (1:250) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.
Lane 1 : Cyclin B1 Recombinant Protein; Lane 2 : Cyclin D1 Recombinant Protein; Lane 3 : Cyclin D2 Recombinant Protein; Lane 4 : Cyclin D3 Recombinant Protein; Lane 5 : Cyclin E1 Recombinant Protein; Lane 6 : Cyclin E2 Recombinant Protein; probed with using bsm-50375M at Cyclin D1 (2G5) Monoclonal Antibody 0.5ug\/mL (1:2000) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-50375M

Applications: WB, IP

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRP (C11F2) Monoclonal Antibody, Unconjugated (bsm-0391M) at 1:400 overnight at 4\u00b0C, followed secondary and DAB staining.
Lane 1: C Reactive Protein (CRP); 40ug loaded into the lane; Probed with CRP (C11F2) Monoclonal Antibody, unconjugated (bsm-0391M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: C Reactive Protein(CRP) probed with CRP(C11F2) Monoclona Antibody, Unconjugated (bsm-0391M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U-2OS cell lysates; Lane 2: Human Siha cell lysates probed with CRP(C11F2) Monoclonal Antibody, Unconjugated bsm-0391MR) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0391M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33193m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (bsm-60761R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (bsm-60761R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (bsm-60761R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (bsm-60761R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60761r-100ul

Applications: IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p85 Monoclonal Antibody, Unconjugated (bsm-52408R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p85 Monoclonal Antibody, Unconjugated (bsm-52408R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p85 Monoclonal Antibody, Unconjugated (bsm-52408R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p85 Monoclonal Antibody, Unconjugated (bsm-52408R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52408r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAPDH Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAPDH Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon\r\n; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAPDH Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon\r\n; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAPDH Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52262r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (3F5) Monoclonal Antibody, Unconjugated (bsm-33218M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (3F5) Monoclonal Antibody, Unconjugated (bsm-33218M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (3F5) Monoclonal Antibody, Unconjugated (bsm-33218M) at 1:2000 overnight at 4\u00b0C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with STAT3 monoclonal antibody, unconjugated (bsm-33218M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33218m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-0945m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-0107m-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: Cy3

Catalog #1: bsm-0107m-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: Cy7

Catalog #1: bsm-1240M-FITC

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-1240M-Biotin

Applications: IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-0387m-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-0387m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-2000M-Cy3

Applications: IF(IHC-P)

Reactivity: Virus, Others

Conjugate: Cy3

Catalog #1: bsm-51186M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Jurkat lysate (20\u00b5g) probed with bsm-51210M CYP1A1 (10G3) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: Siha cell lysates; Lane 2: A549 cell lysates probed with CYP1A1 (10G3) Monoclonal Antibody, Unconjugated (bsm-51210M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51210M

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Lane 1: K562 Cell lysates; probed with LTF (119CT80.1.1) Monoclonal Antibody, unconjugated (bsm-51024M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells, stained with LTF (119CT80.1.1) Monoclonal Antibody (bsm-51024M) at 1:25 dilution, followed by Goat anti-mouse IgG secondary antibody at 1:200 dilution. Immunofluorescence image shows cytoplasm staining in HeLa cells (green), actin (red), and nuclear counter stain with DAPI (blue).

Catalog #1: bsm-51024M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33004M-Biotin

Applications: WB

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-33323m-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PTEN(S380) (4C9) Monoclonal Antibody, Unconjugated (bsm-52192R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PTEN(S380) (4C9) Monoclonal Antibody, Unconjugated (bsm-52192R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PTEN(S380) (4C9) Monoclonal Antibody, Unconjugated (bsm-52192R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Rat Cerebrum tissue lysates; Lane 2: Human SH-SY5Y cell lysates probed with Phospho-PTEN (Ser380) Monoclonal Antibody, Unconjugated (bsm-52192R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52192r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33188m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33188m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33034m-cy3-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-10895m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Lane 1: Mouse Thymus; Probed with CCL5 (6A1) Monoclonal Antibody (bsm-54125R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CCL5 (6A1) Monoclonal Antibody (bsm-54125R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CCL5 (6A1) Monoclonal Antibody (bsm-54125R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CCL5 (6A1) Monoclonal Antibody (bsm-54125R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54125r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated(bsm-54150R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated(bsm-54150R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated(bsm-54150R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated(bsm-54150R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-54150r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52709r

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-54300r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated(bsm-52466R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated(bsm-52466R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated(bsm-52466R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated(bsm-52466R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-52466r-TR

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated