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Published Antibodies


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Catalog #1: bs-4504R

Applications: WB, ELISA

Reactivity: PRRSV

Conjugate: Unconjugated

U2-OS cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD163\/M130 Polyclonal Antibody(bs-2527R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD163\/M130 Polyclonal Antibody,FITC Conjugated(bs-2527R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2527R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Paraformaldehyde-fixed, paraffin-embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes, DAB and counterstaining.
Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Rat Blood Plasma; Lane 2: Rat Blood Plasma; Probed with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Raw264.7 Cells; Probed with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-20373R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 12kD. Observed band size:48kD.\\n
Image provided by the Independent Validation Program (badge number 29759). Lane 1: HeLa cell extract, Lane 2: c6\/36 mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-Caspase 8 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Caspase 8 Polyclonal Antibody (bs-0052R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Caspase 8 Polyclonal Antibody (bs-0052R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-0052R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat transplant lymphoma labeled with Anti-Caspase-1 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF-7 Cells lysates probed with Caspase-1 P10 Polyclonal Antibody, unconjugated (bs-0169R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Caspase-1 P10 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: U-87MG Cells; 30ug loaded into the lane; Probed with Caspase-1 P10 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-0169R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human HT-29 lysates probed with Rabbit Anti-Glut2 Polyclonal Antibody, Unconjugated (bs-0351R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Hep G2 cells probed with GLUT2 Polyclonal Antibody, unconjugated (bs-0351R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Lane 1: Mouse embyo lysates probed with GLUT2 Polyclonal Antibody, Unconjugated (bs-0351R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0351R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Unconjugated

Catalog #1: bs-0295p-cy5-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-15509R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human 293T cells probed with CD32B(Tyr292) Polyclonal Antibody, unconjugated (bs-6031R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD32B(Tyr292) Polyclonal Antibody, Unconjugated (bs-6028R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-6031R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Monkey

Conjugate: Unconjugated

293T cell lysates probed with LAG-3 Polyclonal Antibody, Unconjugated (bs-2646R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
K562 cell lysates probed with LAG-3 Polyclonal Antibody, Unconjugated (bs-2646R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Ctll-2 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lymphocyte Activation Gene 3Antibody(bs-2646R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2646R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti Arginase II Polyclonal Antibody, Unconjugated (bs-11397R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Anti-Arginase II Polyclonal Antibody, Unconjugated (bs-11397R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
DU145 Cell lysates; probed with Arginase II Polyclonal Antibody, unconjugated (bs-11397R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Arginase II) polyclonal Antibody, Unconjugated (bs-11397R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-11397R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-PEGF Polyclonal Antibody, Unconjugated (bs-0731R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat eye, ciliary body labeled Anti-PEGF Polyclonal Antibody, Unconjugated (bs-0731R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-0731R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti CMKLR1 Polyclonal Antibody, Unconjugated (bs-12478R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-CMKLR1 Polyclonal Antibody, Unconjugated (bs-10185R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Huvec (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CMKLR1 Antibody (bs-10185R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Liver tissue lysates probed with CMKLR1 Polyclonal Antibody, Unconjugated (bs-10185R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-10185R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-GAS 6 Polyclonal Antibody, Unconjugated (bs-7549R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse liver lysates Lane 2: Mouse kidney lysates probed with Rabbit Anti-GAS 6 Polyclonal Antibody, Unconjugated (bs-7549R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-7549R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Mouse spleen lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Jurkat cell lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1:U937 cell lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Lung lysates; Lane 4: Mouse Thymus lysates; Lane 5: Mouse RAW264.7 cell lysates; Lane 6: Human THP-1 cell lysates; Lane 7: Human Raji cell lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6934R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Rabbit heart lysates probed with Anti-MMP-2 Polyclonal Antibody, Unconjugated (bs-4599R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-MMP-2 Polyclonal Antibody, Unconjugated (bs-4599R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Anti-MMP-2 Polyclonal Antibody, Unconjugated (bs-4599R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat bladder tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP-2 Polyclonal Antibody, Unconjugated (bs-4599R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-4599R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse hindfoot tumor labeled with Anti-\u00b5-Opioid receptor Polyclonal Antibody, Unconjugated (bs-3623R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse skin labeled with Anti-\u00b5-Opioid receptor Polyclonal Antibody, Unconjugated (bs-3623R) followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3623R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Image provided by Independent Validation Program, badge 29801.\\n\\nLane 1: HeLa G2\/M Phase cell lysate. Lane 2: C6\/36 cell lysates probed with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Antibody, Unconjugated (bs-3332R) at 1:200 for 10 hours at 4˚C. Followed by conjugation to secondary antibody at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated (bs-3332R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Catalog #1: bs-3332R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2399R

Applications: WB, ELISA, IHC-P, IF(IHC-F), IF(ICC)

Reactivity: Human, GuineaPig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated(bs-1992R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (bs-1992R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE)and DAPI staining
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR\/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR\/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1992R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Human HL-60 cells probed with CD15 Polyclonal Antibody, Unconjugated (bs-1702R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Lane 1: MCF-7 lysates probed with CD15\/Fut4\/SSEA-1 Polyclonal Antibody, Unconjugated (bs-1702R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 60 min at 37˚C.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD15\/Fut4\/SSEA-1 Polyclonal Antibody(bs-1702R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1702R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-phospho-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human stomach cancer tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Kindey lysates probed with STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1658R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

\"Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-CSF3\/G-CSF Polyclonal Antibody (bs-1023R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining\\n\"
L1 rat brain, L2 rat heart lysates probed (bs-1023R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band and observed band size: 19kDa
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CSF3 Polyclonal Antibody, Unconjugated (bs-1023R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CSF3 Polyclonal Antibody, Unconjugated (bs-1023R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-1023R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Images provided by the Independent Validation Program (badge number 29749). Lane 1: A549 cell extract Lane 2: c6\/36 mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
HeLa cells probed with PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PALB2) polyclonal Antibody, Unconjugated (bs-0588R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0588R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: Unconjugated

Lane 1: Lovo cell lysates; Lane 2: MCF-7 cell lysates probed with Neurotensin Receptor 1 Polyclonal Antibody, Unconjugated (bs-12002R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: NTSR1 KO Human 293T cell lysates; Lane 2: NTSR1 KO Human 293T cell lysates; Lane 3: Human 293T cell lysates probed with Neurotensin Receptor 1 Polyclonal Antibody, Unconjugated (bs-12002R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12002R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3514R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-11 Polyclonal Antibody, Unconjugated (bs-1827R) at 1:400 overnight at 4\u00b0C, DAB staining.
Mouse cerebellum lysates probed with IL-11 Polyclonal Antibody, Unconjugated (bs-1827R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with IL-11 Polyclonal Antibody, Unconjugated (bs-1827R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1827R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10154R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-7694R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-EAAT1\/GLAST Polyclonal Antibody (bs-1003R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti EAAT1\/Glast Polyclonal Antibody, Unconjugated (bs-1003R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 56kD. Observed band size: 56kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (EAAT1) Polyclonal Antibody, Unconjugated (bs-1003R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (EAAT1) Polyclonal Antibody, Unconjugated (bs-1003R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1003R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse kidney lysates Lane 2: mouse lung lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-2602R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 39kD. Observed band size: 39kD.
Lane 1: HepG2 cell lysates; Lane 2: Mouse Lung lysates; Lane 3: Rat Lung lysates probed with IL10RB Polyclonal Antibody, Unconjugated (bs-2602R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2602R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse liver lysates probed with Rabbit Anti-AQP9 Polyclonal Antibody, Unconjugated (bs-2060R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-AQP9 Polyclonal Antibody, Unconjugated (bs-2060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: Mouse intestine lysates probed with Rabbit Anti-AQP9 Polyclonal Antibody, Unconjugated (bs-2060R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-2060R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2925R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-TPH\/Trptophan Hydroxylase Polyclonal Antibody, Unconjugated (bs-1215R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HL-60 cell lysates probed with Rabbit Anti-Trptophan Hydroxylase Polyclonal Antibody, Unconjugated (bs-1215R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Rat heart lysates probed with Trptophan Hydroxylase Polyclonal Antibody, Unconjugated (bs-1215R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Liver tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates probed with TPH Polyclonal Antibody, Unconjugated (bs-1215R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1215R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1289R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ADRB3\/beta 3 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-1063R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-beta 3 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-1063R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-1063R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-SCF Polyclonal Antibody (bs-0545R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse spleen cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were then stained with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:33 for 30 min at room temperature. A Goat anti-rabbit IgG-AF647 secondary antibody was used at 1ug for 40 min at room temperature. Acquisition of 10,000 events was performed. Primary antibody staining (green) is compared to compared to unstained cells (purple), secondary only (light blue), and isotype control (orange).
Sp2\/0 (Mouse myeloma cell) lysates probed with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
U87MG cells were fixed with 4% PFA (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were then stained with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:100 for 30 min at room temperature. A Goat anti-rabbit IgG-PE (bs-0295G-PE) secondary antibody was used at 1ug for 40 min at room temperature. Acquisition of 20,000 events was performed. Primary antibody staining (green) is compared to compared to unstained cells (purple), secondary only (light blue), and isotype control (orange).

Catalog #1: bs-0545R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0295D-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Formalin-fixed and paraffin embedded human liver cancer labeled with Anti ADCY10 Polyclonal Antibody, Unconjugated (bs-3916R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti ADCY10 Polyclonal Antibody, Unconjugated(bs-3916R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Lane 1: Human U-87 MG cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Jurkat cell lysates probed with ADCY10 Polyclonal Antibody, Unconjugated (bs-3916R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3916R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti- IL-5 Polyclonal Antibody, Unconjugated (bs-1318R) followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-5 Polyclonal Antibody, Unconjugated (bs-1318R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1318R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon labeled with Anti SLC26A4 Polyclonal Antibody, Unconjugated (bs-6787R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human TT cell lysates; Lane 2: Human U251 cell lysates probed with SLC26A4 Polyclonal Antibody, Unconjugated (bs-6787R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6787R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PMP22 Polyclonal Antibody, Unconjugated (bs-0235R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat spinal cord labeled with Anti-PMP22 Polyclonal Antibody, Unconjugated (bs-0235R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Catalog #1: bs-0235R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial carcinoma labeled with Anti-SIRT1 Polyclonal Antibody, Unconjugated (bs-0921R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-SIRT1 Polyclonal Antibody, Unconjugated (bs-0921R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Hela cell lysates probed with Anti-SIRT1 Polyclonal Antibody, Unconjugated (bs-0921R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C.
Protein: mouse heart lysate; Primary: rabbit Anti-SIRT1 (bs-0921R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 58\/81 kDa Observed band size: 58 kDa

Catalog #1: bs-0921R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, FITC Conjugated (bsm-0917M-FITC) at 1:200 for 40 minutes at 37\u00b0C followed by labeling Rabbit Anti-Tubulin Beta, Cy3 conjugated(bs-4511R-Cy3) 1:200, 40 minutes at 37\u00b0C
Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 50kD. Observed band size: 50kD.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1:Mouse heart lysates; Lane 2: Human MCF7 cell lysates probed with Rabbit Anti-Tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-4511R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat testis labeled with Anti-SOX9\/SRA1 Polyclonal Antibody, Unconjugated (bs-4177R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
SW480 and Ht29 lysates probed with SOX9 Polyclonal Antibody, unconjugated (bs-4177R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
U-251 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SOX9 Polyclonal Antibody(bs-4177R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
293T cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SOX9 Antibody(bs-4177R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-4177R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti MT-ND6 Polyclonal Antibody, Unconjugated (bs-3955R) followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MT-ND6 Polyclonal Antibody, Unconjugated (bs-3955R) at 1:400 overnight at 4\u00b0C, DAB staining.
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MT-ND6 Polyclonal Antibody(bs-3955R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3955R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-SHH Polyclonal Antibody, Unconjugated (bs-1544R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse embryo lysates Lane 2: rat brain lysates probed with Anti ShhPolyclonal Antibody, Unconjugated (bs-1544R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 19\/50kD. Observed band size: 50kD.\\n
Human A549 cells probed with Rabbit Anti-Shh Polyclonal Antibody, Unconjugated (bs-1544R) (green) at 1:10 for 30 minutes followed by a FITC conjugated secondary antibody compared to control cells (blue).
This image was generously submitted by Dr. Guo from Yale School of Medicine. Formalin-fixed and paraffin embedded mouse P1 ureter labeled with Rabbit Anti-Shh Polyclonal Antibody, Unconjugated (bs-1544R).

Catalog #1: bs-1544R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-PCX\/PODXL Polyclonal Antibody, Unconjugated (bs-1345R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-PCX\/PODXL Polyclonal Antibody, Unconjugated (bs-1345R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1345r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated