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Staining of canine lymph node tissue with bs-3077r. The primary antibody bs-3077r was used at a 100 fold (A and B) and 500 fold (C and D) dilution. A no primary antibody negative control (E and F) was used to verify the specificity of the stain. The blocking step with avidin and biotin was omitted for the no primary antibody negative controls. As a result, there is some unspecific staining in these sections. \r\nImages in the upper row (A, C, and E) were captured at 10x magnification, while the images below (B, D, and F) were taken at 20x magnification. Image was kindly submitted by a researcher via the Antibodies Online Independent Validation Program. \r\n
Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-MCSF Receptor (Tyr723) Polyclonal Antibody(bs-3077R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3077R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2453R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-IRF7 Polyclonal Antibody, Unconjugated (bs-2994R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-IRF7 Polyclonal Antibody, Unconjugated (bs-2994R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRF7 Antibody(bs-2994R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2994R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat uterus labeled with Anti MAFbx\/Fbx32 Polyclonal Antibody, Unconjugated (bs2591R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Fbx32 Polyclonal Antibody(bs-2591R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Fbx32) Polyclonal Antibody, Unconjugated (bs-2591R) at 1:50 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Fbx32) Polyclonal Antibody, Unconjugated (bs-2591R) at 1:50 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-2591R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human lung carcinoma labeled with EGFR Polyclonal Antibody, Unconjugated (bs-0165R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EGFR Polyclonal Antibody, Unconjugated (bs-0165R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Tissue\/cell: Human Rectal Carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37\u2103 for 20 min; Incubation: EGFR Polyclonal Antibody, Unconjugated (bs-0165R) at 1:200, overnight at 4\u00b0C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml, blue) was used to stain the cell nuclei.
Human HUVEC Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 0.5% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min. Cells were stained with EGFR Polyclonal Antibody, Unconjugated (bs-0165R) at 1:100 for 30 min on ice. The secondary antibody, Goat anti-rabbit IgG-PE, was used for 30 min on ice. Primary Antibody staining (green) is compared to unstained cells (dark blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-0165R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:400 overnight at 4\u00b0C, DAB staining.
Mouse cerebllumlysates probed with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-23376R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8561R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1:rat brain lysates probed with Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (bs-6695R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse Testis lysates probed with Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (bs-6695R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat testis\uff09; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytochrome P450 17A1) Polyclonal Antibody, Unconjugated (bs-6695R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Rat Adrenal gland tissue lysates probed with Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (bs-6695R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6695R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Mouse, Rat, Others

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GDF9 Polyclonal Antibody, Unconjugated (bs-4720R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Ovary cell lysates probed with GDF9 Polyclonal Antibody, Unconjugated (bs-4720R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GDF9 Polyclonal Antibody(bs-4720R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-4720R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse heart lysates probed with SPHK1 Polyclonal Antibody, unconjugated (bs-2652R) at 1:30 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n
Lane 1: Mouse Pancreas tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Rat Kidney tissue lysates probed with SPHK1 Polyclonal Antibody, Unconjugated (bs-2652R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2652R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse heart lysates Lane 2: mouse brain lysates probed with Anti ERK1\/2(p42\/p42 MAPK) Polyclonal Antibody, Unconjugated (bs-2637R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD.
A549 cell lysates probed with Rabbit Anti-ERK1 + 2 Polyclonal Antibody, Unconjugated (bs-2673R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Lane 1: Mouse Kidney lysates; Lane 2: Mouse Liver lysates probed with ERK1 + 2 Polyclonal Antibody, Unconjugated (bs-2637R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A431 cell Lysate;Lane 2: Mouse Cerebrum Lysate;Lane 3: Rat Cerebrum Lysate;Lane 4: Mouse Lymph node Lysate;Lane 5: Rat Lymph node Lysate;Lane 6: SiHa cell Lysate;Lane 7: HUVEC cell Lysate probed with ERK1 + ERK2 Polyclonal Antibody, Unconjugated (bs-2637R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C\r\n\r\n

Catalog #1: bs-2637R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse bone lysates probed with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
HEK 293 stained with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:500 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Catalog #1: bs-20297R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-1351P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm. \\n
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Granzyme B Polyclonal Antibody, Unconjugated (bs-1351R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Granzyme B Antibody(bs-1351R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1351R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: U2os lysates; Lane 2: MDA-MB-231 lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-11929R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: 293T cell lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-11929R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n

Catalog #1: bs-11929R

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Cyclin B1 Polyclonal Antibody, Unconjugated (bs-0572R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HeLa cells probed with Rabbit Anti-Cyclin B1 Polyclonal Antibody (bs-0572R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.\\n
HeLa lysates probed with Anti \u2013(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.
U937 lysates probed with Anti \u2013(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0572R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-SPR Polyclonal Antibody, Unconjugated (bs-0064R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti Substance P Receptor\/NK1R Polyclonal Antibody, Unconjugated (bs-0064R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.
HeLa lysates probed with Neurokinin 1 Receptor Polyclonal Antibody, unconjugated (bs-0064R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
U251 lysates probed with Neurokinin 1 Receptor Polyclonal Antibody, unconjugated (bs-0064R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-0064r-TR

Applications: WB, ELISA

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0699R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti- Scavenger receptor class B member 1 Polyclonal Antibody, Unconjugated (bs-1186R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Image kindly submitted by Brittany Baldwin-Murphy from Cornell University.\\n\\nChick embryo liver hepatocytes probed with Rabbit Anti- Scavenger receptor class B member 1 Polyclonal Antibody at 1:100 or 1:500.\\n
Mouse liver cells probed with Rabbit Anti- Scavenger receptor class B member 1 Polyclonal Antibody (bs-1186R) at 1:100 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG PE conjugated secondary (bs-0295G-PE) at 1:100 (green) for 40 minutes compared to control cells (blue).
HepG2 lysates probed with Scavenger receptor class B member 1 Polyclonal Antibody, unconjugated (bs-1186R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-1186R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat stomach labeled with Rabbit Anti-ACVR2B\/ACTR-IIB Polyclonal Antibody, Unconjugated (bs-12417R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1:K562 cell lysates; Lane 2: 293T cell lysates probed with ACVR2B\/ACTR2B Polyclonal Antibody, Unconjugated (bs-12417R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Activin Receptor Type IIB) Polyclonal Antibody, Unconjugated (bs-12417R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Mouse Kidney tissue lysates; Lane 5: Human K562 cell lysates; Lane 6: Human SH-SY5Y cell lysates; Lane 7: Human Jurkat cell lysates; Lane 8: Human MOLT4 cell lysates probed with Activin Receptor Type IIB Polyclonal Antibody, Unconjugated (bs-12417R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12417R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse spleen and bone lysates probed with Rabbit Anti-SIRP Alpha Polyclonal Antibody, Unconjugated (bs-2708R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Human U937 probed with \\tSIRP Alpha Polyclonal Antibody (bs-2708R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Mouse Cerebrum lysates probed with SIRP Alpha Polyclonal Antibody, Unconjugated (bs-2708R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Brain Lysates; Lane 2: Mouse Cerebellum Lysates; Lane 3: Mouse Spleen Lysates; Lane 4: Mouse Placenta Lysates; Lane 5: Rat Brain Lysates; Lane 6: Rat Cerebellum Lysates. Probed with SIRP Alpha polyclonal Antibody, unconjugated (bs-2708R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2708R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0813R-HRP

Applications: WB, ELISA, IHC-F

Reactivity: Bovine, Cow

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CENPA Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CENPA Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CENPA Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CENPA Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2753R

Applications: ELISA, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1867R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti hospho-DDX5 (Tyr593) Polyclonal Antibody, Unconjugated (bs-8005R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti hospho-DDX5 (Tyr593) Polyclonal Antibody, Unconjugated (bs-8005R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-8005R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti-TNFSF15 Polyclonal Antibody, Unconjugated (bs-5092R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-TNFSF15 Polyclonal Antibody, Unconjugated (bs-5092R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Mouse kidney and bone lysates probed with TNFSF15 Polyclonal Antibody, unconjugated (bs-5092R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.\\n

Catalog #1: bs-5092R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0947R-FITC

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Lane 1: MCF-7 Lane 2: DU145 lysates probed with Anti-Estrogen Receptor alpha Polyclonal Antibody, Unconjugated (bs-0122R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Tissue\/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0122R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Rat Liver lysates; Lane 4: Human MOLT-4 cell lysates; Lane 5: Human Raji cell lysates; Lane 6: Human Hela cell lysates; Lane 7: Human A549 cell lysates probed with Estrogen Receptor alpha Polyclonal Antibody, Unconjugated (bs-0122R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0122R

Applications: WB, ELISA, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-6692R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse placenta labeled with Rabbit Anti-AMIGO2 Polyclonal Antibody, Unconjugated (bs-11450R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cell lysates probed with AMIGO2 Polyclonal Antibody, Unconjugated (bs-11450R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AMIGO2) Polyclonal Antibody, Unconjugated (bs-11450R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AMIGO2) Polyclonal Antibody, Unconjugated (bs-11450R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-11450R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse liver lysates Lane 2: mouse intestine lysates probed with Anti AHSG\/Fetuin A\/Alpha 2 HS Glycoprotein Polyclonal Antibody, Unconjugated (bs-2922R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 31kD. Observed band size: 31kD.
Antigen: bs-2922P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: Mouse Liver lysates probed with AHSG Polyclonal Antibody, Unconjugated (bs-2922R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2922R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Nociceptin Polyclonal Antibody, Unconjugated(bs-0075R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 rat liver lysates probed (bs-0075R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 19kDa.

Catalog #1: bs-0075R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-5977R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3689R

Applications: WB, ELISA, FCM, IHC-P

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung cancer labeled with Rabbit Anti-SGK1 (Thr256) Polyclonal Antibody, Unconjugated(bs-3397R) 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Mouse pancreas lysates probed with Phospho-SGK1 (Thr256) Polyclonal Antibody, Unconjugated (bs-3397R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3397R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2905R

Applications: ELISA, IHC-P, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with SLC26A6 Polyclonal Antibody, Unconjugated \\tbs-20817R at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SLC26A6 Polyclonal Antibody, Unconjugated (bs-20817R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SLC26A6 Polyclonal Antibody, Unconjugated (bs-20817R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20817R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat ovary tissue labeled with Anti-Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-1032R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-1032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 39kD. Observed band size: 39kD

Catalog #1: bs-1032R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6597R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti phospho-Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti-Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Raw264.7 cell lysates probed with Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3012R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2601R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human pancreatic cancer labeled with Anti-EDG2\/LPA1 Polyclonal Antibody, Unconjugated (bs-2880R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
L1 Mouse liver lysates, L2 Mouse cerebrum lysates probed with Anti- EDG2\/LPA1 Polyclonal Antibody, Unconjugated (bs-2880R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:2000 90min in 37˚C. Predicted band and observed band size: 41kD
Lane 1: Mouse Heart lysates probed with LPAR1 Polyclonal Antibody, Unconjugated (bs-2880R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with LPA1\/LPAR1 Polyclonal Antibody, Unconjugated (bs-2880R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2880R

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0961R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-NCAM1 Polyclonal Antibody, Unconjugated (bs-0736R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Human HeLa lysates probed with Rabbit Anti-CD56 Polyclonal Antibody, Unconjugated (bs-0736R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0736R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33281M

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Mouse spleen lysates probed with Rabbit Anti-GPX1 Polyclonal Antibody, Unconjugated (bs-3882R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.
Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Rat Liver tissue lysates; Lane 5: Rat Heart tissue lysates; Lane 6: Rat Spleen tissue lysates; Lane 7: Rat Kidney tissue lysates probed with GPX1 Polyclonal Antibody, Unconjugated (bs-3882R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3882r-TR

Applications: WB, ELISA, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Anti-IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2993R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Bdkrb2\/B2R Polyclonal Antibody, Unconjugated (bs-2422R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse brain cells probed with Rabbit Anti-Bdkrb2 Polyclonal Antibody (bs-2422R) at 1:50 for 40 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue).

Catalog #1: bs-2422R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti- 5-HTR3Polyclonal Antibody, Unconjugated(bs-2126R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebellum lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Spinal cord lysates; Lane 4: Mouse Heart lysates ; Lane 5: Rat Heart lysates probed with 5-HTR3\/HTR3A Polyclonal Antibody, Unconjugated (bs-2126R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2126R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated