TM9SF1 Polyclonal Antibody

MCF-7 lysates probed with TM9SF1 Polyclonal Antibody, Unconjugated (bs-10764R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Lane 1: Kindey lysates probed with TM9SF1 Polyclonal Antibody, Unconjugated (bs-10764R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Human Jurkat cell lysates; Lane 2: Human MCF-7 cell lysates probed with TM9SF1 Polyclonal Antibody, Unconjugated (bs-10764R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Jurkat cell lysates; Lane 2: Human MCF-7 cell lysates probed with TM9SF1 Polyclonal Antibody, Unconjugated (bs-10764R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Horse
Rabbit

Overview
Catalog #	bs-10764R
Product Name	TM9SF1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TM9SF1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10548
Swiss Prot	O15321
Subcellular location	Cytoplasm, Cell membrane
Synonyms	HMP 70; HMP70; MP 70; MP70; Multispanning membrane protein (70kD); Transmembrane 9 superfamily member 1; Transmembrane protein 9 superfamily member 1; TM9S1_HUMAN.
Background	Plays an essential role in autophagy.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200