MOBP Polyclonal Antibody

Lane 1: Mouse Brain lysate; Probed with MOBP Polyclonal Antibody, Unconjugated (bs-11184R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-MOBP Polyclonal Antibody, Unconjugated (bs-11184R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\\n$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-MOBP Polyclonal Antibody, Unconjugated (bs-11184R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\\n

$Positive sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat brian, left); Negative sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat liver, right);\r\nAntigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MOBP) Polyclonal Antibody, Unconjugated (bs-11184R) at 1:200 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.\r\n$

Positive sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat brian, left); Negative sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat liver, right);\r\nAntigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MOBP) Polyclonal Antibody, Unconjugated (bs-11184R) at 1:200 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.\r\n

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-11184R
Product Name	MOBP Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MOBP
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4336
Swiss Prot	Q13875
Subcellular location	Cytoplasm
Synonyms	Myelin-associated oligodendrocyte basic protein; MOBP
Background	May play a role in compacting or stabilizing the myelin sheath, possibly by binding the negatively charged acidic phospholipids of the cytoplasmic membrane.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200