ZNF423 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-ZNF423\/OAZ Polyclonal Antibody, Unconjugated (bs-12220R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-ZNF423\/OAZ Polyclonal Antibody, Unconjugated (bs-12220R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue Lysates; Lane 4: Rat Cerebellum tissue Lysates; Lane 5: Human SH-SY5Y cell Lysates probed with ZNF423 Polyclonal Antibody, Unconjugated (bs-12220R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue Lysates; Lane 4: Rat Cerebellum tissue Lysates; Lane 5: Human SH-SY5Y cell Lysates probed with ZNF423 Polyclonal Antibody, Unconjugated (bs-12220R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-12220R
Product Name	ZNF423 Polyclonal Antibody
Applications	ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity	This antibody may also detect the protein Midasin if used in Human.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ZNF423/OAZ
Immunogen Range	901-1000/1284
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23090
Swiss Prot	Q2M1K9
Subcellular location	Nucleus
Synonyms	OAZ; Roaz; hOAZ; Ebfaz; JBTS19; NPHP14; ZFP423; Zfp104; Zinc finger protein 423; Olf1/EBF-associated zinc finger protein; Smad- and Olf-interacting zinc finger protein; ZNF423; KIAA0760
Background	Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200