SMAD1 Polyclonal Antibody

Formalin-fixed and paraffin embedded human breast cancer labeled with SMAD1 Polyclonal Antibody, Unconjugated (bs-16376R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Line 1, mouse heart lysates\uff1bline 2, mouse muscle lysates; probed with SMAD1 Polyclonal Antibody, Unconjugated (bs-16376R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.$

Line 1, mouse heart lysates\uff1bline 2, mouse muscle lysates; probed with SMAD1 Polyclonal Antibody, Unconjugated (bs-16376R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Chicken

Overview
Catalog #	bs-16376R
Product Name	SMAD1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This antibody may cross react with AKAP6 due to a few animo acid sequence similarity.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SMAD1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4086
Swiss Prot	Q15797
Subcellular location	Cytoplasm, Nucleus
Synonyms	BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1
Background	Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200