ABCB11 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat pancreatic tissue labeled with Anti-BSEP\/ABCB11 Polyclonal Antibody, Unconjugated (bs-1954R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat pancreatic tissue labeled with Anti-BSEP\/ABCB11 Polyclonal Antibody, Unconjugated (bs-1954R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BSEP ) Polyclonal Antibody, Unconjugated (bs-1954R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BSEP ) Polyclonal Antibody, Unconjugated (bs-1954R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BSEP ) Polyclonal Antibody, Unconjugated (bs-1954R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BSEP ) Polyclonal Antibody, Unconjugated (bs-1954R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF

Reactivity

Predicted Reactivity

Human
Mouse
Rabbit

Overview
Catalog #	bs-1954R
Product Name	ABCB11 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse BSEP
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	27413
Swiss Prot	Q9QY30
Subcellular location	Cell membrane
Synonyms	Bsep; PGY4; SPGP; ABC16; Lith1; PFIC2; Bile salt export pump; ATP-binding cassette sub-family B member 11; Sister of P-glycoprotein; Abcb11
Background	Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F
IF