CPSF4 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-CPSF4\/CPSF30 Polyclonal Antibody, Unconjugated (bs-2253R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-CPSF4\/CPSF30 Polyclonal Antibody, Unconjugated (bs-2253R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti CPSF4\/CPSF30 Polyclonal Antibody, Unconjugated (bs-2253R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 27kD. Observed band size: 23kD, 27kD.$

Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti CPSF4\/CPSF30 Polyclonal Antibody, Unconjugated (bs-2253R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 27kD. Observed band size: 23kD, 27kD.

Applications

WB
ELISA
IHC-P
IHC-F

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-2253R
Product Name	CPSF4 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CPSF4
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10898
Swiss Prot	O95639
Subcellular location	Nucleus
Synonyms	NAR; NEB1; CPSF30; Cleavage and polyadenylation specificity factor subunit 4; Cleavage and polyadenylation specificity factor 30 kDa subunit; CPSF 30 kDa subunit; NS1 effector domain-binding protein 1; Neb-1; No arches homolog; CPSF4
Background	Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500