SMAD1 + SMAD5 Polyclonal Antibody

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with SMAD1 + SMAD5 Polyclonal Antibody, Unconjugated (bs-2973R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with SMAD1 + SMAD5 Polyclonal Antibody, Unconjugated (bs-2973R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-2973R
Product Name	SMAD1 + SMAD5 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF, ICC/IF
Specificity	The sequence used to produce this antibody is conserved in Smad 1, 2, 3 and 9 and expected to cross react due to sequence homology.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SMAD5
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	4090
Swiss Prot	Q99717
Subcellular location	Cytoplasm, Nucleus
Synonyms	DWFC; JV5-1; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; SMAD family member 5; SMAD 5; Smad5; hSmad5
Background	Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Application Dilution
WB	1:500-2000
IHC-P	1:100-500
IHC-F	IFICC1:100
IF
ICC/IF