ADAM12 Polyclonal Antibody

Mouse heart lysates probed with ADAM12 Polyclonal Antibody, Unconjugated (bs-5847R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$U-87MG cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM12 Antibody\uff08bs-5847R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).$

U-87MG cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM12 Antibody\uff08bs-5847R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Pig
Chicken
Rabbit

Overview
Catalog #	bs-5847R
Product Name	ADAM12 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Pig, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ADAM12
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8038
Subcellular location	Secreted, Cell membrane
Synonyms	A disintegrin and metalloproteinase domain 12; A disintegrin and metalloproteinase domain 12; ADA12_HUMAN; ADAM 12; ADAM metallopeptidase domain 12; ADAM12; Disintegrin and metalloproteinase domain-containing protein 12; MCMP; MCMPMltna; MCMPMltna; Meltrin alpha; Meltrin-alpha; MLTN; MLTNA; OTTHUMP00000046766.
Background	Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400