CYPA Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-CYPA Polyclonal Antibody, Unconjugated (bs-5912R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Lymph node tissue lysates; Lane 3: Mouse Lung tissue lysates; Lane 4: Mouse NIH\/3T3 cell lysates; Lane 5: Rat Thymus tissue lysates; Lane 6: Rat Lymph node tissue lysates; Lane 7: Rat Lung tissue lysates; Lane 8: Human Hela cell lysates; Lane 9: Human PANC-1 cell lysates; Lane 10: Human Molt-4 cell lysates; Lane 11: Human HUVEC cell lysates; Lane 12: Human THP-1 cell lysates probed with CYPA Polyclonal Antibody, Unconjugated (bs-5912R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Lymph node tissue lysates; Lane 3: Mouse Lung tissue lysates; Lane 4: Mouse NIH\/3T3 cell lysates; Lane 5: Rat Thymus tissue lysates; Lane 6: Rat Lymph node tissue lysates; Lane 7: Rat Lung tissue lysates; Lane 8: Human Hela cell lysates; Lane 9: Human PANC-1 cell lysates; Lane 10: Human Molt-4 cell lysates; Lane 11: Human HUVEC cell lysates; Lane 12: Human THP-1 cell lysates probed with CYPA Polyclonal Antibody, Unconjugated (bs-5912R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Rabbit

Overview
Catalog #	bs-5912R
Product Name	CYPA Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Cyclophilin A.
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5478
Swiss Prot	P62937
Subcellular location	Cytoplasm
Synonyms	CYPA; CYPH; HEL-S-69p; Peptidyl-prolyl cis-trans isomerase A; PPIase A; Cyclophilin A; Cyclosporin A-binding protein; Rotamase A; PPIA
Background	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200