DATF1 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human glioma labeled with Rabbit Anti DATF1\/IDO1 Polyclonal Antibody, Unconjugated (bs-7659R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human glioma labeled with Rabbit Anti DATF1\/IDO1 Polyclonal Antibody, Unconjugated (bs-7659R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Anti-DATF1\/IDO1 Polyclonal Antibody, Unconjugated(bs-7659R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C$

Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Anti-DATF1\/IDO1 Polyclonal Antibody, Unconjugated(bs-7659R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Horse
Chicken

Overview
Catalog #	bs-7659R
Product Name	DATF1 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IDO1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	11083
Swiss Prot	Q9BTC0
Subcellular location	Cytoplasm, Nucleus
Synonyms	BYE1; DIO1; DATF1; DIDO2; DIDO3; DIO-1; DATF-1; C20orf158; dJ885L7.8; Death-inducer obliterator 1; hDido1; Death-associated transcription factor 1; DIDO1; KIAA0333
Background	Putative transcription factor, weakly pro-apoptotic when overexpressed (By similarity). Tumor suppressor. Required for early embryonic stem cell development. Isoform 2: Displaces isoform 4 at the onset of differentiation, required for repression of stemness genes.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200