Tropomyosin Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin-embedded Human Myocardial tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with Pan-Tropomyosin Polyclonal Antibody, Unconjugated (bs-9622R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.$

Paraformaldehyde-fixed, paraffin-embedded Human Myocardial tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with Pan-Tropomyosin Polyclonal Antibody, Unconjugated (bs-9622R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Sheep
Zebrafish

Overview
Catalog #	bs-9622R
Product Name	Tropomyosin Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Specificity	This antibody will detect all 4 Tropomyosin proteins: TPM1, TPM2, TPM3, and TPM4
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Sheep, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TPM1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7168
Swiss Prot	P09493
Subcellular location	Cytoskeleton
Synonyms	TPM1; Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1; C15orf13; TMSA; TPM2; Tropomyosin beta chain; Beta-tropomyosin; Tropomyosin-2; TMSB; TPM3; Tropomyosin alpha-3 chain; Gamma-tropomyosin; Tropomyosin-3; TPM4; Tropomyosin alpha-4 chain; TM30p1; Tropomyosin-4
Background	Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200